Plasmid

Part:BBa_I735003:Design

Designed by: Giovanni Russo   Group: iGEM07_Naples   (2007-09-14)


Cloning CDS PHO80 in psB3C6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 2044
    Illegal XbaI site found at 2278
    Illegal XbaI site found at 2381
    Illegal XbaI site found at 2938
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2044
    Illegal NheI site found at 863
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2050
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2044
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2
    Illegal EcoRI site found at 2044
    Illegal XbaI site found at 2278
    Illegal XbaI site found at 2381
    Illegal XbaI site found at 2938
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2044
    Illegal XbaI site found at 2278
    Illegal XbaI site found at 2381
    Illegal XbaI site found at 2938
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 453
    Illegal AgeI site found at 777
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2880


Design Notes

We needed to mutate CDS BBa_I735002


Source

The plasmid used is BBa_J23019 and the CDS is BBa_I735002 (mutated)

References