Plasmid
Part:BBa_I735003:Design
Designed by: Giovanni Russo Group: iGEM07_Naples (2007-09-14)
Cloning CDS PHO80 in psB3C6
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 1
Illegal EcoRI site found at 2044
Illegal XbaI site found at 2278
Illegal XbaI site found at 2381
Illegal XbaI site found at 2938 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2044
Illegal NheI site found at 863
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2050 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2044
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 2
Illegal EcoRI site found at 2044
Illegal XbaI site found at 2278
Illegal XbaI site found at 2381
Illegal XbaI site found at 2938 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2044
Illegal XbaI site found at 2278
Illegal XbaI site found at 2381
Illegal XbaI site found at 2938
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 453
Illegal AgeI site found at 777 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2880
Design Notes
We needed to mutate CDS BBa_I735002
Source
The plasmid used is BBa_J23019 and the CDS is BBa_I735002 (mutated)